Fig. 3.

Circ_0001875 promotes the tumorigenesis and metastasis of NSCLC cells in vivo. a Representative images of a subcutaneous tumor nude mouse model. Hypodermic injection of A549 cells stably transfected with lentivirus expressing the circ-0001875 overexpression construct (pLCDH-circ-0001875) or control vector (pLCDH-vector) was performed in nude mice. b Images of subcutaneous xenograft tumors (n = 9 for each group). c Tumor weight of the two groups. d Tumor volume was measured every week. e–f HE and Ki67 IHC staining of xenograft tumors, images are shown at 400 × magnification. Scale bar = 50 μm. g Representative images of HE staining in tail vein–injected mouse models (n = 9 for each group), images are shown at 100 × magnification. Scale bar = 100 μm. h The numbers of lung and liver metastatic tumors were counted. Data are shown as the means ± SD. *p < 0.05, **p < 0.01, ***p < 0.001