Table 3.

Antibodies for flow cytometric analysis on CiGEnCs.

Target	Activated C3	C4d	C5b-9	CD59
Primary antibody	Monoclonal Mouse anti-human activated C3 antibody, which recognizes C3b, iC3b, and C3c fragments (Clone bH6, HM2168S, Hycult biotech, Uden, The Netherlands) 1:50	Mouse anti-human C4d antibody (Clone 12D11, HM2229 20UG, Hycult biotech, Uden, The Netherlands) 1:100	Mouse anti-human neoantigen-C9 antibody (HM2264, Hycult biotech, Uden, The Netherlands) 1:100	Mouse anti-human CD59 (HM2120, Hycult biotech, Uden, The Netherlands) 1:100
Secondary antibody	Goat anti-mouse FITC-labelled antibody (Cat. No. 1031-02, SouthernBioTech, Uden, The Netherlands) 1:100	Goat anti-mouse FITC-labelled antibody (Cat. No. 1031-02, SouthernBioTech, Uden, The Netherlands) 1:100	Goat anti-mouse FITC-labelled antibody (Cat. No. 1031-02, SouthernBioTech, Uden, The Netherlands) 1:100	Goat anti-mouse FITC-labelled antibody (Cat. No. 1031-02, SouthernBioTech, Uden, The Netherlands) 1:100

Incubation with primary antibodies was performed on ice for 30 min. Incubation with secondary antibodies was conducted in dark for an additional time of 30 min.