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. Author manuscript; available in PMC: 2022 Apr 27.
Published in final edited form as: Nat Struct Mol Biol. 2022 Jan 10;29(1):47–58. doi: 10.1038/s41594-021-00706-2

Extended Data Fig. 3 |. IMPDH2-Wt filament resists gtP inhibition.

Extended Data Fig. 3 |

a,b, GTP inhibition curves of IMPDH2 or IMPDH1-WT (solid line) and the respective non-assembly y12A protein (dashed line). Individual data points are shown as diamonds (IMPDH2) or circles (IMPDH1), where filled are WT and empty y12A. reactions were performed in triplicate and the average for each concentration is shown as a bold rectangle (filled is WT, empty is y12A). Error bars are standard deviation calculated from n = 3. reactions performed with 1 μM protein, 1 mM ATP, 1 mM IMP, 300 μM nAD+, and varying GTP.