Figure 3.

Memory B cells colocalize with infected cells early after activation

(A) Point patterns of BRM cell distribution before and after rechallenging. Plots display clustering L values of observed data versus complete spatial random simulation (simulation n = 1,000).

(B) L values at r = 200 of BRM cells. Data are pooled from 3–5 independent experiments per group. Each circle represents the mean L value calculated for one mouse (± SD), based on multiple images tiles collected from each mouse.

(C) BRM cells 24 h post rechallenge with CFP-S-Flu. Dotted lines demarcate border between highly infected and uninfected areas. Cells are highlighted using Imaris-created spots.

(D) BRM cell density in infected and uninfected sites 24 h after rechallenge. Each pair of points represents the average BRM cell density in one mouse, obtained by averaging data from multiple large tiles per animal.

(E) A representative image from mice treated with PTX 2 h after CFP-S-Flu rechallenge.

(F) L value plot of BRM cells in PTX-treated rechallenged mice.

(G) BRM cell density in infected and uninfected areas of PTX-treated, rechallenged lungs.

Data are pooled from 4 (C and D) or 3 (E and G) independent experiments. Statistical analyses were made using Mann-Whitney test (B) and paired t tests (D and G). Error bars represent SD. ^∗p < 0.5; ^∗∗p < 0.01. See also Figure S3.