Figure 4.

Plasma cells appear within infected alveoli within 4 days of rechallenge

(A) TPLSM images of infected and uninfected BAT mice 4 days post rechallenge with CFP-S-Flu. PCs are highlighted using Imaris-created spots (yellow).

(B) Alveolar PC density in infected or uninfected areas 4 days after rechallenge with CFP-S-Flu. Data are pooled from 4 independent experiments.

(C) Left, confocal microscopy of alveolar PCs near infected cells. Right, zoom of indicated boxed region.

(D) L values of observed PC positioning compared with spatial random simulation. Data show the results of one experiment out of 5 performed (at least one mouse per group per experiment).

(E) PC L values at r = 200.

(F) Densities of alveolar and clustered PCs. Each circle represents the mean densities measured in one mouse, obtained by averaging data from multiple large tiles per animal.

Data in (E) and (F) are pooled from 3–5 independent experiments. Statistical analyses were made using a paired t test (B), one-way ANOVA (E), and Mann-Whitney U test (F). Error bars represent SD. ^∗∗p < 0.01;^∗∗∗∗p < 0.0001.