REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-B220 AlexaFluor700, Clone RA3-6B2	Biolegend	Cat# 103232; RRID:AB_493717
Anti-Ly6G PE, Clone 1A8	Biolegend	Cat# 127608; RRID:AB_1186099
Anti-CD69 BV711, Clone H1.2F3	Biolegend	Cat# 104537; RRID:AB_2566120
Anti-GL7 AlexaFluor647	Biolegend	Cat# 144606; RRID:AB_2562185
Anti-GL7 Pacific Blue	Biolegend	Cat# 144614; RRID:AB_2563292
Anti-GL7 PerCP/Cy5.5	Biolegend	Cat# 144610; RRID:AB_2562979
Anti-PDL2 PeCy7, Clone B7-DC	Biolegend	Cat# 107214; RRID:AB_2728123
Anti-CD80 BV750, Clone B7-1	BD Biosciences	Cat# 747436; RRID:AB_2872118
Anti-CD73 APC, Clone TY/11.8	Biolegend	Cat# 127210; RRID:AB_11218786
Anti-CXCR3 BV605, Clone S18001A	Biolegend	Cat# 155915; RRID:AB_2892317
Anti-CD45 APC, Clone 30F11	Biolegend	Cat# 103112; RRID:AB_312977
Anti-CD45.1 Pacific Blue,Clone A20	Biolegend	Cat# 110722; RRID:AB_492866
Anti-CD45.2 Pacific Blue, Clone 104	Biolegend	Cat# 109820; RRID:AB_492872
Anti-IgM PECy7, Clone RMM-1	Biolegend	Cat# 406514; RRID:AB_10642031
Anti-IgG1 Biotin, Clone RMG1-1	Bioloegend	Cat# 406604; RRID:AB_315063
Anti-IgG2b Biotin, Clone RMG2b-1	Biolegend	Cat# 406704; RRID:AB_315067
Anti-IgG2a[b] Biotin, Clone 5.7	BD Biosciences	Cat# 553504; RRID:AB_394889
Anti-IgA AlexaFluor647	Southern Biotech	Cat# 1040-31; RRID:AB_2794377
InVivoMAb anti-mouse IFNg, Clone XMG1.2	BioXCell	Cat# BE0055; RRID:AB_1107694
InVivoMAb anti-mouse CD40L, Clone MR-1	BioXCell	Cat# BE0017-1; RRID:AB_1107601
InVivoMAb anti-mouse CXCR3, Clone CXCR3-173	BioXCell	Cat# BE0249; RRID:AB_2687730
InVivoMAb rat IgG2a isotype control, anti-trinitrophenol	BioXCell	Cat# BE0089; RRID:AB_1107769
InVivoMAb Armenian Hamster IgG Isotype Control; anti GST, Clone PIP	BioXCell	Cat# BE0260; RRID:AB_2687739
TotalSeq™-C0914 anti-mouse CD273, Clone B7-DC	Biolegend	Cat# 107229; RRID:AB_2860615
TotalSeq™-C0015 anti-mouse Ly-6G,Clone 1A8	Biolegend	Cat# 127657; RRID:AB_2819863
TotalSeq™-C0810 anti-mouse CD138, Clone 281-2	Biolegend	Cat# 142538; RRID:AB_2860696
TotalSeq™-C0093 anti-mouse CD19, Clone 6D5	Biolegend	Cat# 115571; RRID:AB_2832392
TotalSeq™-C0114 anti-mouse F4/80, Clone BM8	Biolegend	Cat# 123157; RRID:AB_2832437
TotalSeq™-C0228 anti-mouse CXCR3, Clone CXCR3-173	Biolegend	Cat# 126545; RRID:AB_2832454
TotalSeq™-C0197 anti-mouse CD69, Clone H1.2F3	Biolegend	Cat# 104551; RRID:AB_2832333
TotalSeq™-C0120 anti-mouse TCR β chain, Clone H57-597	Biolegend	Cat# 109259; RRID:AB_2819820
TotalSeq™-C0013 anti-mouse Ly-6C, Clone HK1.4	Biolegend	Cat# 128051; RRID:AB_2832461
TotalSeq™-C0014 anti-mouse/human CD11b, Clone M1/70	Biolegend	Cat# 101275; RRID:AB_2832272
TotalSeq™-C0117 anti-mouse I-A/I-E, Clone M5/114.15.2	Biolegend	Cat# 107659; RRID:AB_2832368
TotalSeq™-C0112 anti-mouse CD62L, Clone MEL-14	Biolegend	Cat# 104455; RRID:AB_2819800
TotalSeq™-C0106 anti-mouse CD11c, Clone N418	Biolegend	Cat# 117361; RRID:AB_2819834
TotalSeq™-C0103 anti-mouse/human B220, Clone RA3-6B2	Biolegend	Cat# 103273; RRID:AB_2832307
TotalSeq™-C0301 anti-mouse Hashtag 1, Clone M1/42; 30-F11	Biolegend	Cat# 155861; RRID:AB_2800693
TotalSeq™-C0302 anti-mouse Hashtag 2, Clone M1/42; 30-F11	Biolegend	Cat# 155863; RRID:AB_2800694
TotalSeq™-C0303 anti-mouse Hashtag 3, Clone M1/42; 30-F11	Biolegend	Cat# 155865; RRID:AB_2800695
Purified Goat anti-mouse IgG (minimal x-reactivity), Poly4053	Biolegend	Cat# 405301; RRID:AB_315005
Purified anti-mouse Ig light chain κ Antibody, Clone RMK-12	Biolegend	Cat# 407202; RRID:AB_345326
Mouse Anti-Hamster IgG Antibody, Clone MAH1.12	R and D Systems	Cat# MAB011; RRID:AB_357352
IgG from Mouse Serum	Sigma-Aldrich	Cat# I5381; RRID:AB_1163670
Bacterial and virus strains
X31 influenza; H3N2 strain	Cloned and propagated in house	N/A
CFP-S-Flu; [S-eCFP/N1(PR8)].H1(PR8)	Generated, cloned and propagated in house	N/A
GFP-S-Flu; [S-eGFP/N1(PR8)].H1(PR8)	Provided by Alain Townsend, (Powell et al., 2012)	N/A
Chemicals, peptides, and recombinant proteins
Pertussis Toxin Islet Activating Protein Salt-Free (PTX)	Quadratech Diagnostics Ltd.	Cat# 181
Clodronated liposomes (CLL)	Liposoma BV	Cat# CP-010-010
Control liposomes (PBS)	Liposoma BV	Cat# CP-010-010
Fixable Viability Dye e780	Life Technologies Ltd	Cat# 65-0865-14
Fingolimod (FTY720) HCl	Stratech	Cat# S5002
Collagenase D	Roche	Cat# 11088858001
DNase I	Merck	Cat# DN25
RNase A	Merck	Cat# R4875
Ultra TMB-ELISA Substrate Solution	Thermo Scientific	Cat# 34028
Streptavidin-BV605	Biolegend	Cat# 405229
Streptavidin-Horseradish Peroxidase	Jackson ImmunoResearch	Cat# 016-030-084; RRID: AB_2337238
Biotin-X-NHS	Sigma-Aldrich	Cat# 203188
Critical commercial assays
Cytofix/Cytoperm W/GolgiPlug Kit	BD Biosciences	Cat# 555028, RRID:AB_2869013
MagniSort Mouse B cell Enrichment Kit	Thermo Fisher Scientific	Cat# 8804-6827-74, RRID:AB_2575267
Deposited data
Murine bulk RNA-Seq: lung BRM (ivCD45- B220+ CD69+ GL7- dTomato+ HA+), splenic Bmem (B220+ CD38+ GL7- dTomato+ HA+) and splenic naïve B cell (B220+ CD38+ GL7- dTomato-)	This paper	Gene Expression Omnibus: GSE183135; (https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE183135)
Murine scRNA-Seq: Lung ivCD45- ex-vivo CD45+ cells; d0 resting, D1 X31 rechallenge and D1 X31 rechallenge+CLL treatment	This paper	Gene Expression Omnibus: GSE194058
Experimental models: Organisms/strains
Mouse: Rosa26-tdTomato: B6.Cg-Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J	Jackson Laboratory	Cat# 007909; RRID:IMSR_JAX:007909
Mouse: BLIMP1-mVenus: Prdm1-mVenus	Provided by M. Saitou, (Ohinata et al., 2008)	Riken accession CDB0460T
Mouse: AID-cre: B6.129P2-Aicdatm1(cre)Mnz/J	Jackson Laboratory	Cat# 007770; RRID:IMSR_JAX:007770
Mouse: CD19Cre/Cre: B6.129P2(C)-Cd19tm1(Cre)Cgn/J	Jackson Laboratory	Cat# 006785; RRID:IMSR_JAX:006785
Mouse: Rosa26-stop-YFP: B6.129X1-Gt(ROSA)26Sortm1(EYFP)Cos/J	Jackson Laboratory	Cat# 006148; RRID:IMSR_JAX:006148
Mouse: Ub-GFP: C57BL/6-Tg(UBC-GFP)30Scha/J	Jackson Laboratory	Cat# 004353; RRID:IMSR_JAX:004353
Oligonucleotides
TaqMan Gene Expression Assay. Mm00434946_m1 Cxcl9, FAM-MGB	Life Technologies Ltd	Cat# 4331182
TaqMan Gene Expression Assay. Mm00445235_m1 Cxcl10	Life Technologies Ltd	Cat# 4331182
TaqMan Gene Expression Assay. Mm00441259_g1 Ccl3	Life Technologies Ltd	Cat# 4331182
TaqMan Gene Expression Assay. Mm01302427_m1 Ccl5	Life Technologies Ltd	Cat# 4331182
TaqMan Gene Expression Assay. Mm00443111 Ccl4	Life Technologies Ltd	Cat# 4331182
TaqMan Gene Expression Assay. Mm99999915_g1 Gapdh gene	Life Technologies Ltd	Cat# 4331182
Software and algorithms
Flowjo, v10.8, Treestar Inc.	https://www.flowjo.com/	RRID: SCR_008520
Graphpad Prism v9	https://www.graphpad.com/scientificsoftware/	RRID: SCR_002798
Adobe Illustrator CS6	http://www.adobe.com/products/ illustrator.html	RRID: SCR_010279
Imaris v9.2.1	http://www.bitplane.com/imaris/imaris	RRID: SCR_007370
Zen Digital Imaging for Light Microscopy	http://www.zeiss.com/microscopy/en_us/products/microscope-software/	RRID: SCR_013672
R Studio	https://rstudio.com/	RRID:SCR_000432