FIG 3.
Immune response after vaccination in HFH4-hACE2 mice. (A and B) Sera were collected from infected vaccinated mice, and the PRNT50 values against SARS-CoV-2, rWIV1, and rRsSHC014S were determined. (C) An IFN-γ ICS assay was performed using the indicated viral structural protein peptide pools. (D) Gating strategy used to analyze T-cell responses. Splenocytes were gated for lymphocytes (FSC-A/SSC-A), single cells (FSC-A/FSC-H), CD3+ (APC-Cy7/SSC-A), and CD4+ or CD8+ (PerCP-Cy5.5/FITC) cells, followed by populations expressing IFN-γ. Error bars indicate standard errors. Statistical significance was assessed using Wilcoxon’s matched-pairs signed rank test (A and B) and two-tailed Student t tests compared to the no-pep group (C) (*, P < 0.05).