TABLE 1.

Phenotypes of HIV-1_AD8 Env variants with changes in Gln 114 and Gln 567^a

Env	Env processing	Infectivity (%)	Resistance/sensitivity compared to wild type			gp120-trimer association
			Cold	sCD4	BNM-III-170
Wild type	●	●	●	●	●	●
Q114A	●	●	●	●	●	+
Q114D	+	●	RR	RR	RR	++
Q114E	+	+	RR	RR	RR	++
Q114K	None	ND	ND	ND	ND	NA
Q114N	●	ND	ND	ND	ND	●
Q114S	●	ND	ND	ND	ND	●
Q567A	●	+	●	●	●	●
Q567E	●	− − −	●	●	●	●
Q567K	+	●	R	R	R	++
Q567R	+	−	Slight R	●	●	+
Q114A Q567K	●	●	Slight R	R	R	++
Q114A Q567R	●	●	●	●	●	+
Q114D Q567R	+	●	R	RR	RR	++
Q114E Q567A	+	+	RR	RR	RR	++
Q114E Q567K	+	●	RRR	RRR	RRR	++
Q114E Q567E	+	−	R	●	R	++
Q114E Q567R	+	●	RR	RR	RR	++
Q114K Q567E	None	ND	ND	ND	ND	NA

^aThe phenotypes of the wild-type HIV-1_AD8 Env and the indicated Gln 114 and Gln 567 variants were determined as described in the legend to Fig. 2. The values for Env processing efficiency, virus infectivity, and gp120-trimer association, relative to those observed for the wild-type HIV-1_AD8 Env, are shown. The sensitivity or resistance of viruses with the Env variants to cold, sCD4, and BNM-III-170 is reported relative to that of the wild-type HIV-1_AD8 virus. To ensure accurate comparison of the Env variant phenotypes across multiple assays, the wild-type HIV-1_AD8 and key Env mutants (e.g., Q114E or Q567K) were included in all assays. Phenotypes are labeled as follows: •, wild-type level; +, increase; −, decrease; R, resistant; S, sensitive; ND, not determined; NA, not applicable. For virus infectivity: − − −, 0 to 25% of wild-type level; −, 51 to 75%; •, 76 to 125%; +, >125%. An increase in the number of R symbols indicates a greater level of resistancecompared to the wild-type level. The levels of gp120-trimer association exhibit the following order: ++ > + > •, where • indicates the wild-type level. The data shown are representative of results obtained in at least two independent experiments.