TABLE 2.
Fecal coronavirus screening informationa
| Sample | RdRp1 | RdRp2 | Spike1 | Spike2 | Spike3 | Helicase | Location | Accession no.b |
|---|---|---|---|---|---|---|---|---|
| CP-20-01 | Jefferson County, TN, USA | MZ220722–MZ220724 | ||||||
| CP-20-02 | Washington County, TN, USA | MZ220725–MZ220727 | ||||||
| CP-20-03 | TN, USA | NA | ||||||
| CP-20-04 | Monroe County, TN, USA | MZ220728–MZ220730 | ||||||
| CP-20-05 | TN, USA | NA | ||||||
| CP-20-06 | Sullivan County, TN, USA | NA | ||||||
| CP-20-07 | Grainger County, TN, USA | MZ220731–MZ220734 | ||||||
| CP-20-08 | Monroe County, TN, USA | MZ220735 | ||||||
| CP-20-09 | Sullivan County, TN, USA | MZ220736–MZ220738 | ||||||
| CP-20-10 | Washington County, TN, USA | NA | ||||||
| CP-20-11 | Jefferson County, TN, USA | NA | ||||||
| CP-20-12 | Monroe County, TN, USA | MZ220739–MZ220742 | ||||||
| CP-20-13 | Grainger County, TN, USA | MZ220743–MZ220745 | ||||||
| CP-20-14 | Jefferson County, TN, USA | NA | ||||||
| CP-20-15 | Sullivan County, TN, USA | NA | ||||||
| CP-20-16 | Sevier County, TN, USA | NA | ||||||
| CP-20-17 | Cocke County, TN, USA | NA | ||||||
| CP-20-18 | Hamblin County, TN, USA | NA | ||||||
| CP-20-19 | Washington/Sullivan County, TN, USA | MZ220746–MZ220747 | ||||||
| CP-20-20 | Hamblin County, TN, USA | MZ220748 | ||||||
| CP-20-21 | Washington/Sullivan County, TN, USA | NA | ||||||
| CP-20-22 | Grainger County, TN, USA | NA | ||||||
| CP-20-23 | Jefferson County, TN, USA | MZ220749–MZ220750 | ||||||
| CP-20-24 | Jefferson County, TN, USA | NA | ||||||
| CP-20-25 | Grainger County, TN, USA | NA | ||||||
| CP-20-26 | Grainger County, TN, USA | MZ220751–MZ220755 | ||||||
| CP-20-27 | Grainger County, TN, USA | MZ220756–MZ220759 | ||||||
| CP-20-28 | Washington/Sullivan County, TN, USA | MZ220760 | ||||||
| CP-20-29 | TN, USA | NA | ||||||
| CP-20-30 | Grainger County, TN, USA | MZ220761–MZ220762 |
a
Sample IDs were assigned to deidentified fecal samples from healthy cats. RNA was extracted from each and PCR screened for amplification of 6 coronavirus loci across 3 conserved genes (RdRp, spike, and helicase). Positive amplification and sequencing are shown by a black shaded box. Out of 30 samples, 15 were positive for at least 1 coronavirus loci. Animals originated from multiple East Tennessee counties, and when known, the exact county of origin is listed. Accession numbers for each sample are shown to the right.
b
NA, not applicable, no amplification.