FIG 1.

NgR1 is dispensable for reovirus spread to the brain and replication at that site following peroral inoculation. (A) NgR1 expression in brain-tissue lysates of WT and NgR1^−/− mice at postnatal days 4 (P4) and 10 (P10) were determined by immunoblotting using an antibody specific for mouse NgR1 (AF1440). Vinculin was used as a loading control. (B and C) WT, JAM-A^−/−, NgR1^−/−, and DKO mice were inoculated perorally with 10⁴ PFU of reovirus T3SA−. Titers in the (B) intestine and (C) brain at the indicated intervals are shown. n = 8 or 9 animals per group for each time point. Error bars indicate standard errors of the means (SEM). Titers in tissues from knockout animals were compared with those in WT mice at each time point. **, P < 0.01; ***, P < 0.001; ****, P < 0.0001 (two-way analysis of variance [ANOVA] with Dunnett’s multiple-comparison test).