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. 2022 Apr 4;10(2):e02421-21. doi: 10.1128/spectrum.02421-21

FIG 1.

FIG 1

E-cigarette aerosols reduce the growth of S. sanguinis and S. gordonii. (A) Overnight cultures of S. sanguinis were diluted 1:10 into e-cig aerosol-pretreated medium. OD600 was measured every 5 min over the course of 8 h. The average was calculated and plotted for OD600 (top) and log2 transform (bottom). (B) Slopes for linear regression of S. sanguinis are presented based on the derivative of the log2 transform. (C) Overnight cultures of S. gordonii were diluted 1:10 into e-cig aerosol-pretreated medium. Optical density was measured every 5 min over the course of 16 h. The average was calculated and plotted OD600 (top) and log2 transform (bottom). (D) Slopes for linear regression of S. gordonii are presented based on the derivative of the log2 transform. Data are representative of one biological (n = 3) replicate repeated at least twice. OD600 of treatment groups was compared to control OD600, using one-way ANOVA (Dunnett’s correction). All conditions were compared to the control; for a complete breakdown of significance, refer to Table 1.