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. 2022 Apr 27;18(4):e10824. doi: 10.15252/msb.202110824
Reagent or resource Reference or source Identifier or catalog number
Biological samples and experimental models
Melbourne unexposed healthy control Walter and Eliza Hall Institute of Medical Research Volunteer Blood Donor Registry N/A
Timika healthy community control Pigapu and Hirapau villages, Timika HC

Timika asymptomatic

P. falciparum malaria

 Pigapu and Hirapau villages, Timika AM

Timika symptomatic

P. falciparum malaria

 Rumah Sakit Mitra Masyarakat Hospital, Timika SM
C57BL6/J (M. musculus) Jex Laboratory, Walter and Eliza Hall Institute N/A
Antibodies
ELISA antibodies
Fc‐HRP‐conjugated mouse anti‐human IgG Southern Biotech

Clone H2;

Cat# 9042‐05
Surface marker antibodies for CYTOF
141Pr‐conjugated mouse anti‐human CD196/CCR6 Fluidigm

Clone 11A9;

Cat# 3141014A
144Nd‐conjugated mouse anti‐human CD45RA Fluidigm

Clone HI100;

Cat# 3143006B
146Nd‐conjugated mouse anti‐human IgD Fluidigm

Clone IA6‐2;

Cat# 3146005B
147Sm‐conjugated mouse anti‐human CD20 Fluidigm

Clone 2H7;

Cat# 3147001B
151Eu‐conjugated hamster anti‐human ICOS Fluidigm

Clone DX29;

Cat# 3151020B
152Sm‐conjugated mouse anti‐human CD21 Fluidigm

Clone BL13;

Cat# 3152010B
153Eu‐conjugated rat anti‐human CXCR5 Fluidigm

Clone RF8B2;

Cat# 3153020B
156Gd‐conjugated mouse anti‐human CXCR3 Fluidigm

Clone G025H7;

Cat# 3156004B
158Gd‐conjugated mouse anti‐human CD10 Fluidigm

Clone HI10a;

Cat# 3158011B
165Ho‐conjugated mouse anti‐human CD19 Fluidigm

Clone HIB19;

Cat# 3165025B
167Er‐conjugated mouse anti‐human CD27 Fluidigm

Clone L128;

Cat# 3167006B
170Er‐conjugated mouse anti‐human CD3 Fluidigm

Clone UCHT1;

Cat# 3170001B
172Yb‐conjugated mouse anti‐human IgM Fluidigm

Clone MHM‐88;

Cat# 3172004B
174Yb‐conjugated mouse anti‐human CD4 Fluidigm

Clone SK3;

Cat# 3174004B
175Lu‐conjugated mouse anti‐human PD‐1 Fluidigm

Clone EH12.2H7;

Cat# 3175008B
176Yb‐conjugated mouse anti‐human CD127 Fluidigm

Clone A019D5;

Cat# 3176004B
APC‐conjugated rat anti‐human IgG Biolegend

Clone M1310G05;

Cat# 410711
PE‐conjugated mouse anti‐human FcRL5 Biolegend

Clone 509f6;

Cat# 340304
145Nd‐conjugated mouse anti‐human PE Fluidigm

Clone PE001;

Cat# 3145006B
162Dy‐conjugated mouse anti‐human APC Fluidigm

Clone APC003;

Cat# 3162006B
161Dy‐conjugated mouse anti‐human T‐bet Fluidigm

Clone 4B10;

Cat# 3161014B
Infection model antibodies
Syrian hamster anti‐mouse CTLA‐4 Walter and Eliza Hall Institute Clone 9H10
Ultra‐LEAF™ Purified Armenian Hamster IgG Isotype Control Biolegend

Clone 400959;

Cat# 400969
Flow cytometry antibodies
AF700‐conjugated mouse anti‐human CD3 Biolegend

Clone UCHT1;

Cat# 300424
BV786‐conjugated mouse anti‐human CD4 BD Biosciences

Clone SK3;

Cat# 664528
BV421‐conjugated mouse anti‐human CD19 Biolegend

Clone HIB19;

Cat# 302234
PE/Cy7‐conjugated mouse anti‐human CD25 Biolegend

Clone M‐A251;

Cat# 356108
BV711‐conjugated mouse anti‐human PD‐1 Biolegend

Clone EH12.2H7;

Cat# 329928
BV650‐conjugated mouse anti‐human CD45RA Biolegend

Clone HI100;

Cat# 304136
APC/Fire750‐conjugated mouse anti‐human CD8 Biolegend

Clone SK1;

Cat# 980914
PE/Dazzle594‐conjugated mouse anti‐human CTLA‐4 Biolegend

Clone BNI3;

Cat# 369616
BB700‐conjugated mouse anti‐human FoxP3 BD Biosciences

Clone 236A/E7;

Cat# 566526
Rat anti‐mouse CD16/CD32 Fc Block BD Biosciences

Clone 2.4G2;

Cat# 553142
FITC‐conjugated hamster anti‐mouse TCR β chain BD Biosciences Clone H57‐597; Cat# 553170
PE/Cy7‐conjugated rat anti‐mouse CD4 Biolegend

Clone RM4‐5;

Cat# 100528
BV785‐conjugated anti‐mouse CD44 Biolegend

Clone IM7;

Cat# 103041
PerCp‐Cy5.5‐conjugated rat anti‐mouse CXCR5 BD Biosciences

Clone 2G8;

Cat# 560528
BV421‐conjugated Armenian hamster anti‐mouse CXCR3 Biolegend

Clone CXCR3‐173;

Cat# 126521
APC/Cy7‐conjugated rat anti‐mouse CD62L Biolegend

Clone MEL‐14;

Cat#104428
Biotin‐conjugated rat anti‐mouse CCR7 Biolegend

Clone 4B12;

Cat# 120103
Pacific Blue™‐conjugated rat anti‐mouse FoxP3 Biolegend

Clone MF‐14;

Cat# 126409
APC‐conjugated Armenian hamster anti‐mouse CTLA‐4 Biolegend

Clone UC10‐4B9;

Cat# 106309
Chemicals, Enzymes, and other reagents
P. falciparum lysate (5 μg/ml) Generated in‐house N/A
P. falciparum recombinant protein (0.5–2 μg/ml) Generated in‐house N/A
1x105 P. chabaudi chabaudi AS Generated in‐house N/A
Tetramethyl‐benzidine/H2O2 KPL Inc. Cat# 50‐76‐03
Cell‐ID Cisplatin (5 μM) Fluidigm Cat# 201064
Dulbecco’s phosphate‐buffered saline (DPBS) Gibco Cat# 14190144
Human TruStain FcX Biolegend Cat# 422302
Bovine serum albumin (0.5%) (w/v) Sigma‐Aldrich Cat# A7906‐100G
Sodium azide 0.02% (w/v) Sigma‐Aldrich Cat# S8032‐25G
Maxpar nuclear antigen staining buffer set Fluidigm Cat# 201063
Maxpar fix and perm buffer Fluidigm Cat# 201067
Cell‐ID intercalator (125nM) Fluidigm Cat# 201192A
EQ Four Element beads Fluidigm Cat# 201078
Fixable Viability Dye efluor‐506 eBioscience Cat# 65‐0866
Software
CyTOF software v7.0.8493 Fluidigm N/A
FlowJo v10 BD Biosciences https://www.graphpad.com/
Cytobank (Kotecha et al, 2010) https://www.beckman.com/flow‐cytometry/software/cytobank‐enterprise
GraphPad Prism v8.0 GraphPad https://www.graphpad.com/
Rstudio v4.0.3‐4.1.2 (R Core Team, 2021) https://www.R‐project.org/
Rsubread v2.0.1 (Liao et al, 2019) (Liao et al, 2013) http://bioconductor.org/packages/release/bioc/html/Rsubread.html
limma v3.46.0 (Ritchie et al, 2015) http://bioconductor.org/packages/release/bioc/html/limma.html
Ingenuity Pathway Analysis QIAGEN http://pages.ingenuity.com/rs/ingenuity/images/IPA_data_sheet.pdf
Reagent kits and Instruments
QIAamp DNA Blood Mini Kit QIAGEN Cat# 51104
Chameleon plate reader Hidex N/A
Helios mass cytometer Fluidigm N/A
Isolate II RNA Mini Kit Bioline Cat# BIO‐52072
Illumina TruSeq RNA Library Prep Kit (< 100 ng) Illumina Cat# RS‐122‐2001
FoxP3/Transcription Factor Staining Buffer Set eBioscience Cat# 00‐5521‐00
BD LSR Fortessa X20 eBioscience N/A
Agilent TapeStation 2200 Agilent N/A
Illumina NextSeq 500 Illumina N/A