TABLE 1.

Primers used in this study

Primersa	Sequence (5′–3′)b	Restriction site
HA-NCL-F HA-NCL-R HA-NCL (546-699)-F HA-NCL (1-545)-R NCL-GFP-F NCL-GFP-R Fibrillarin-GFP-F Fibrillarin-GFP-R	TGGCCATGGAGGCCCGAATTCGGATGGCTCCTCCTCCAAAGGAGGTG GATCCCCGCGGCCGCGGTACCTTATTCAAACTTCGTCTTCTTTCC TGGCCATGGAGGCCCGAATTCGGGGACCCAGGGGATCGCCTAATG GATCCCCGCGGCCGCGGTACCTTATTGTAACTCCAGCCTGATTGT GAGGTTTAAACTACGGGATCCAATGGCTCCTCCTCCAAAGGAGGTG ACCGGTAGCGCTAGGACGCGTAATTCAAACTTCGTCTTCTTTCCT GAGGTTTAAACTACGGGATCCAATGAAGCCAGGTTTCAGCCCCC ACCGGTAGCGCTAGGACGCGTAAGTTCTTCACCTTCGGAGGCGG	EcoRI KpnI EcoRI KpnI BamHI MluI BamHI MluI

^aF denotes forward primer; R denotes reverse primer.

^bRestriction sites are underlined.