FIG 2.

Strategy for construction of gene deletion vector and identification of VdHP1 mutants. (A) The strategy for homologous recombination of VdHP1 in V. dahliae. (B) and (C) The confirmation of deletion and complemented strains by PCR. (D) Southern hybridization analysis. Probe: Hyg; DNA of WT, ΔVdHP1-1, and ΔVdHP1-2 were digested by HindIII. (E) Phenotype of all mutants on PDA at 10 dpi. Bar = 1cm. (F) The conidia of the morphology of WT, ΔVdHP1, and C-ΔVdHP1 strains under scanning electron micrographs. Scale bar = 4 μm.