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Spiking recovery in sputum samples by Oxford Nanopore (ONT) and by qPCR. Recovery of spiked organisms (H. influenzae, S. pneumoniae, S. aureus, P. aeruginosa, M. Catarrhalis) on metagenomic sequencing using Oxford Nanopore, expressed as (a) % target bacterial genome coverage breadth and (c) % bacterial reads; or (b) detected by pathogen specific PCR.
