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Metal/enzyme ratio and catalytic efficiencies of the FEZ-1 zinc β-lactamase as a function of pHa
| pH | Buffer | kcat (s−1) | Km (μM) | kcat/Km (μM−1 s−1) | Zn/enzyme |
|---|---|---|---|---|---|
| 5 | Sodium acetate | Inactivation | Inactivation | Inactivation | 1.5 |
| 6 | Sodium cacodylate | 320 | 50 | 6.4 | 1.8 |
| 7 | HEPES | 250 | 60 | 4.1 | 1.8 |
| 8 | HEPES | 310 | 64 | 4.8 | 1.5 |
The substrate used for this experiment was cefuroxime. Standard deviations were between 10 and 20%.
