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. 2022 Mar 17;10(2):e02049-21. doi: 10.1128/spectrum.02049-21

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Copyright © 2022 Gou et al.

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FIG 2 — Depletion of lung macrophages counteracts the antibacterial and anti-inflammatory effects of IL-6. Lung macrophages were depleted by intraperitoneal administration of clodronate-liposomes. (A) F4/80⁺ macrophages in BALF after depletion (n = 6 to 7/group). Pneumococcal loads for (B) BALF (n = 7 to 10/group), (C) lungs (n = 7 to 9/group), (D) spleens (n = 5 to 7/group), and (E) livers (n = 5 to 7/group). (F) Hematoxylin and eosin (H&E) staining of lung tissue sections (n = 5/group) and (G) total protein in BALF (n = 4 to 5/group). Black arrow, inflammatory cell infiltration; blue arrow, epithelial cell shedding; red arrow, bleeding.