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. 2022 Mar 17;10(2):e02049-21. doi: 10.1128/spectrum.02049-21

FIG 6.

FIG 6

IL-6 regulates cell death in cultured RAW264.7 cells and alveolar macrophages through GSDME- and GSDMD-mediated pyroptosis. (A) Photomicrographs of RAW264.7 cells in the presence and absence of S. pneumoniae 24 hpi with or without recombinant mouse IL-6 protein (20 ng/mL) as indicated (n = 3/group). (B) RAW264.7 cell death was assessed by lactate dehydrogenase (LDH) release (n = 4/group). (C to D) Caspase-3 (n = 3 to 5/group), GSDME (n = 3 to 6/group), and IL-18 (n = 5 to 12/group) in lysates of S. pneumoniae-infected RAW264.7 cells at the indicated times. (E) IL-1β (n = 6/group) and TNF-α (n = 5/group) in the supernatants of S. pneumoniae-infected RAW264.7 cells at 24 hpi, with or without IL-6 protein, as above. (F) GSDME (n = 3 to 8/group) and GSDMD (n = 3 to 6/group) in the cell lysates of S. pneumoniae-infected alveolar macrophages at the indicated times.