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IL-6 deficiency causes aggravated lung injury due to increased GSDME- and GSDMD-mediated pyroptosis in vivo. IL-1β and TNF-α in (A) lung homogenates (n = 5/group) and (B) BALF (n = 5/group) at 48 and 72 hpi. (C and D) Protein levels of caspase-3 (n = 4/group), GSDME (n = 4/group), and IL-1β (n = 4/group); and (E and F) caspase-1 (n = 4/group), GSDMD (n = 4/group), and IL-18 (n = 4/group) in lungs of mice at 48 and 72 hpi, as indicated.
