TABLE 2.
Characteristics and antibiotic resistance profile of strains harboring ESBL and mcr-1 genes
| Strain | Isolate source/locationa | Bacteria species | Resistance profileb |
MIC | Gene(s) | MLST | |||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AMX | AMC | TZP | KF | CRO | FEP | ETP | IMP | AMK | GEN | CIP | FOF | NIT | DOX | SXT | CST | ||||||
| EC1 | Hospital/GH | E. coli | R | I | S | R | R | R | S | S | S | S | I | S | S | R | R | R | 16 μg/mL | blaTEM-135; blaCTX-M1; mcr-1 | ST648 |
| EC2 | Hospital/GH | E. coli | R | I | S | R | R | I | S | S | S | S | I | S | S | R | R | R | 8 μg/mL | blaTEM-135; blaCTX-M1; mcr-1 | ST648 |
| EC3 | Hospital/GH | E. coli | R | I | S | R | R | R | S | S | S | S | I | S | S | R | R | R | 16 μg/mL | blaTEM-159; blaCTX-M1; mcr-1 | ST648 |
| EC4 | Hospital/GH | E. coli | R | I | S | R | R | I | S | S | S | S | I | S | S | R | R | R | 8 μg/mL | blaTEM-135; blaCTX-M1; mcr-1 | ST648 |
| EC5 | Hospital/EH | E. coli | R | I | S | R | R | R | S | S | S | S | I | S | S | R | R | R | 8 μg/mL | blaTEM-126; blaCTX-M1; mcr-1 | ST648 |
| EC6 | Hospital/GH | E. coli | R | I | S | R | R | I | S | S | S | S | I | S | S | R | R | R | 16 μg/mL | blaCTX-M1; mcr-1 | ST648 |
| EC7 | Hospital/GH | E. coli | R | I | S | R | R | I | S | S | S | S | I | S | S | R | R | R | 16 μg/mL | blaCTX-M1; mcr-1 | ST648 |
| EC8 | Hospital/EH | E. coli | S | I | S | R | R | I | S | S | S | S | S | S | S | S | S | S | bla CTX-M15 | ST2914 | |
| EC9 | Hospital/EH | E. coli | R | R | S | R | R | R | S | S | S | S | I | S | S | S | S | S | bla CTX-M15 | ST2914 | |
| EC10 | Hospital/GH | E. coli | R | I | S | R | R | I | S | S | S | S | I | S | S | R | R | R | 8 μg/mL | blaTEM-159; mcr-1 | ST648 |
| EC11 | Hospital/GH | E. coli | R | I | S | R | R | R | S | S | S | S | I | S | S | R | R | R | 8 μg/mL | blaTEM-1; mcr-1 | ST648 |
| KP1 | Hospital/GH | K. pneumoniae | R | I | S | R | R | R | S | S | S | R | S | S | S | R | R | S | blaTEM-117; blaCTX-M15 | ST551 | |
| EC12 | Collective catering/GH | E. coli | R | R | S | R | R | R | S | S | S | S | S | S | S | R | R | S | blaTEM-128; blaCTX-M15 | ST8149 | |
| EC13 | Collective catering/EH | E. coli | R | I | S | R | R | R | S | S | S | S | S | S | S | R | R | S | bla CTX-M15 | ST8149 | |
| KP2 | Collective catering/GH | K. pneumoniae | R | S | S | R | R | R | S | S | S | S | I | S | S | R | R | S | bla CTX-M15 | ST2010 | |
| KP3 | Collective catering/GH | K. pneumoniae | R | I | S | R | R | R | S | S | S | S | I | S | S | R | R | S | bla CTX-M15 | ST2010 | |
| EC14 | House/GH | E. coli | R | R | S | R | R | R | S | S | S | S | R | S | S | S | S | S | bla CTX-M15 | ST155 | |
aEH, external homogenate; GH, gut homogenate.
MX, amoxicillin; AMC, amoxicillin-clavulanic acid; TZP, piperacillin-tazobactam; KF, cephalothin; CRO, ceftriaxone; FEP, cefepime; ETP, ertapenem; IMP, imipenem; AMK, amikacin; GEN, gentamicin; CIP, ciprofloxacin; FOF, fosfomycin; NIT, nitrofurantoin; DOX, doxycycline; SXT, trimethoprim-sulfamethoxazole; CST, colistin; R, resistant; I, intermediate; S, susceptible; MLST, multilocus sequence typing. Genes: CTX-M, especially blaCTX-15, conferring resistance to all the penicillins and cephalosporins and high-level resistance to other classes of antibiotics, especially fluoroquinolones and cotrimoxazole, but not carbapenems; TEM, conferring resistance to penicillin family antibiotics such as ampicillin and aminopenicillins (especially blaTEM-1/135); MCR-1, conferring resistance to colistin.