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The binding specificity of anti-S2 mAbs. (A) The recombinant SARS-CoV-2 S, NTD, RBD, and S2 proteins or (B) the recombinant S proteins of SARS-CoV-2, SARS-CoV, and MERS-CoV were utilized as the antigens in the ELISA experiments for analyzing the binding specificity of mAbs S2-10H, S2-4D, S2-5D, S2-8D, and S2-8A. Absorbance was measured at 450 nm by a spectrophotometer. Data are presented as means ± SD of three biological replicates (n = 3).
