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The human sera from COVID-19 patients contain S(1127-1167)-specific antibodies. The sfGFP-S(1127-1167)-His (#10) recombinant proteins (2 μg) were separated on SDS-PAGE and subjected to Western blot analysis with anti-His tag MAb (1:2,000) and the human sera (1:1,000) randomly collected from 20 hospitalized COVID-19 patients (P1-P20) within 14–21 days since they were diagnosed with SARS-CoV-2 infection by RT-qPCR.
