TABLE 3.

False-positive species with relative abundance (RA) greater than 1%

Mock community	Related species’ theoretical abundance		MIrROR		16_mar		rrn_DBv2
	Species	RA (%)	Species	RA (%)	Species	RA (%)	Species	RA (%)
MOCK1	L. fermentum	18.4					L. helveticus	2.6
							L. ginsenosidimutans	1.1
	None						Salinicoccus halodurans	3.3
							Shigella boydii	3.2
							Streptococcus pneumoniae	1.7
							Staphylococcus capitis	1.6
MOCK2	V. rogosae	15.9	V. dispar	6.2	V. parvula	6.9	Veillonella sp.	1.9
			V. parvula	3	Veillonella sp. oral clone AA050	5.1	V. atypica	1.8
	P. corporis	5	P. fusca	4.1	P. fusca	7.8	P. fusca	1.8
			P. jejuni	2.8	P. melaninogenica	1.5	P. intermedia	1.3
			Prevotella sp. 000688375	1.3			P. melaninogenica	1.3
			P. intermedia	1.1
	F. nucleatum	15.9			Fusobacterium sp.	1.1
	C. difficile	2.6			C. difficile shuttle vector pDSW1728	1
	B. fragilis	10					B. ovatus	8.3
							B. uniformis	7.8
							B. fragilis	3.1
	None				Subdoligranulum sp. DJF_VR33k2	7.1
					Ruminococcaceae bacterium D16	5.1
							Propionibacterium propionicum	4.9
							Unclassified bacterium	2.9
							Streptococcus pneumoniae	2.8
							Bacillus thuringiensis	1.9
							Vibrio parahaemolyticus	1.8
							Rhodococcus erythropolis	1.5
							Klebsiella pneumoniae	1.3
							Geobacillus sp.	1
							Burkholderia cenocepacia	1