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. 2022 Mar 7;10(2):e02097-21. doi: 10.1128/spectrum.02097-21

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Copyright © 2022 Choudhury et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 6 — Co-localization of SVA 3D mutants with NLRP3 domains. PK-15 cells were transfected with EV together with Myc-NLRP3, Flag-3D together with myc-NLRP3, EV together with Myc-LRR, Flag-3D together with myc-LRR, EV together with Myc-NACHT, Flag-3D together with Myc-NACHT, EV together with Myc-PYD, Flag-3D together with Myc-PYD, EV together with Flag-3D-12, Flag-3D-12 together with Myc-NLRP3, EV together with Flag-3D-13, and Flag-3D-13 together with Myc-NLRP3. Subcellular localization was observed by confocal microscopy. The Pearson’s correlation coefficient was analyzed using the Image-J (Java 1.8.0_172) software. The scale bar was 10 μm.