FIG 3.

VdMRTF1 is required for resistance to Ca²⁺ in Verticillium dahliae. (A) The wild-type, ΔVdMRTF1, and ΔVdMRTF1-C strains were cultured in PDA, PDA with 0.4M CaCl₂, and 0.6M CaCl₂ at 25°C for 10 days. Photographs were taken at 10 dpi. Bar, 2 cm. (B) The bar chart showed the inhibition rate of the wild-type, ΔVdMRTF1, and ΔVdMRTF1-C strains under different concentrations of CaCl₂. Error bars represent the standard deviations based on three independent replicates. Asterisks indicate significant differences (*P < 0.05).