FIG 5.
VdMRTF1 is involved in eliminating endogenous and exogenous reactive oxygen species in Verticillium dahliae. (A) The hyphae of all stains were stained with diaminobenzidine 3,3′-diaminobenzidine (DAB) overnight and observed by light microscopy (DM2500, Leica). Bar = 20 μm. (B) Tobacco seedling roots infected with the wild-type, ΔVdMRTF1, and ΔVdMRTF1-C strains at 7 dpi. Controls (CK) were mock-inoculated with distilled water. The nitroblue tetrazolium (NBT) and DAB stains were used to visualized O2.- and H2O2, respectively.