FIG 7.

VdMRTF1 regulates expression of genes involved in melanin biosynthesis in Verticillium dahliae. (A) Heatmap indicating the FPKM value of genes in a secondary metabolism gene cluster, which are involved in melanin biosynthesis in V. dahliae. The average FPKM value was from three biological repetitions. TBtools was used to normalize data and generate a heat map. The pink color indicates expression was relatively higher in the ΔVdMRTF1 compared with the wild-type VdLs.17 strain, while the blue color indicates expression was relatively lower. (B) The wild-type, VdMRTF1 mutant, and complemented strains were cultured in complete medium (CM), CM with 5 μg/mL, and CM with 15 μg/mL tricyclazole at 25°C for 10 days. Photographs were taken at 10 dpi. Bar, 2 cm.