TABLE 2.

Substrate specificity and kinetic parameters of KDC4427

Substrates	Km (mM)a	kcat (s−1)a	kcat/Km (mM−1 s−1)	Amino acid	%b
Phenylpyruvic acidc	0.60 ± 0.02	186.94 ± 0.39	311.76	Phe	100
2-Ketobutanoic acid	0.66 ± 0.08	185.77 ± 3.08	255.09	Glu	81.82
Indole-3-pyruvic acidc	0.015 ± 0.002	3.7 ± 0.6	247	Trp	79.23
2-Ketopentanoic acid	0.65 ± 0.02	128.77 ± 2.35	198		63.51
2-Ketohexanoic acid	0.52 ± 0.01	78.84 ± 0.51	152.25		48.84
3-Methyl-2-ketobutanoic acid	0.31 ± 0.07	34.88 ± 1.86	115.55	Val	37.06
3-Methyl-2-ketopentanoic acid	0.72 ± 0.06	29.42 ± 0.86	40.95	Ile	13.14
Pyruvic acid	0.86 ± 0.15	22.31 ± 2.79	26.1	Ala	8.37

^aAll data represent mean ± standard deviation of three replicates.

^bk_cat/K_m of phenylpyruvate was taken as the percentage.

^cThe data of PPA and IPyA were cited from our unpublished paper.