TABLE 5.

Detection of Delta lineage in wastewater samples using the Orf8_119del test

Sample	CoV19 E	S157del	Orf8119del
September to December 2020a,b
S-2480	32	NA	NA
S-2493	33	NA	NA
S-2498	30	NA	NA
S-2501	29	NA	NA
S-2505	31	NA	NA
S-2509	33	NA	NA
July to August 2021a,b
12689	28	29	31
12694	28	29	31
12698	30	31	32
12733	28	29	31
12737	26	27	30
12741	28	28	31
12745	29	30	32
12754	27	28	30
12762	30	30	32
12766	29	29	32
Env_128	31	29	32
Env_129	33	31	34
Env_130	31	30	32
Env_134	33	30	34
Env_138	32	29	31
Env_140	30	28	31
Env_143	31	30	33
Env_144	29	28	30
Env_145	30	29	31
Env_146	36	33	33
Env_152	35	30	33
Env_163	29	30	32
Env_168	32	29	32
Env_172	33	32	35
Env_173	30	31	34
Env_177	30	32	34
Env_186	30	31	34
Env_197	29	29	32
Env_198	30	30	33
S-12686	31	31	34

^aRandomly selected wastewater samples that were previously tested with the Alpha-Delta assay were subsequently examined using the confirmatory Orf8_119del test, and the Cq values for each reaction are shown.

^bNA, no amplification.