FIG 1.

Parvimonas micra clinical isolation scheme and transformation assay. (A) (i) Odontogenic abscess samples were collected into anaerobic transport medium and (ii) grown on selective/differential agar PMM. Putative colonies were (iii) confirmed morphologically and (iv) submitted for 16S rRNA sequencing to verify species identity. (B) Wild-type strain ATCC 33270 (wt rif^S) was grown on rifampin containing sBHI medium to isolate a spontaneous rifampin resistance strain (rif^R). The deduced amino acid sequences of the wild-type rif^S and rif^R RpoB proteins are shown. The rif^R mutation is shown in red font. (C) Plate transformation assays were performed by the addition of (i) DNA and (ii) P. micra to sBHI agar. (iii) After 24 h of growth, bacteria were collected into sBHI medium and plated on sBHI medium supplemented with the appropriate antibiotic.