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. 2022 Apr 13;10(2):e00465-22. doi: 10.1128/spectrum.00465-22

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Copyright © 2022 Higashi et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 2 — Insertion of resistance cassette into P. micra clinical isolates. (A) Mutagenesis construct (top) was inserted into the P. micra chromosome through homologous recombination (middle) yielding erythromycin-resistant transformants (bottom). The primers used for PCR verification of genotypes are illustrated by red arrows. (B) Insertion of the erythromycin resistance cassette (∼1 kb) was confirmed by PCR for a selected transformant (ermR A28). (C) Transformation frequencies of P. micra wild-type strains. Transformation frequencies are expressed as the ratio of erythromycin-resistant CFU to total CFU. Values represent the averages from triplicate independent determinations ± standard deviation (SD). “n.d.” indicates a transformation efficiency below the detection limit of the assay.