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Comparison of homology versus transformation efficiency in P. micra. (A) Mutagenesis constructs were generated with flanking regions of various sizes. (B) Wild-type clinical isolates A28 and A1 were transformed with the mutagenesis constructs illustrated in panel A. Values are averaged from triplicate independent determinations ± SD. “n.d.” indicates a transformation efficiency below the detection limit of the assay.
