FIG 5.

Green renilla luciferase (RenG) expression in P. micra. (A) Genomic context and sequence of a constitutively expressed renG transcription fusion to the tuf gene. The tuf gene Rho-independent terminator is indicated by a ball and stick icon. (B) Luciferase activity was measured in wild-type strain A28 and the luciferase reporter strain (c-renG). Normalized luciferase activity (RLU) was calculated by dividing raw luciferase values by the culture OD₆₀₀ values. (C) Correlation between viable cell counts (CFU) and luciferase reporter values in the constitutive luciferase reporter strain c-renG (R² = 0.99). All data points represent the average values ± SD from triplicate independent determinations. Statistical analysis was performed using a two-tailed Student’s t test. *, P < 0.05.