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. 2022 Apr 13;10(2):e00465-22. doi: 10.1128/spectrum.00465-22

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Copyright © 2022 Higashi et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 7 — In vitro mariner transposon mutagenesis in P. micra. (A) Purification of recombinant MarC9 transposase and (B) the mini-mariner transposon plasmid pT-MGL-erm. (C) One microgram of P. micra gDNA was mutagenized using 0.125, 0.25, 0.5, and 1.0 μg of recombinant MarC9 transposase with a fixed quantity of the mini-mariner transposon. These reactions were subsequently transformed into wild-type P. micra strain A28. The transformation results are shown on the graph and by (D) growth on erythromycin supplemented media. Data are expressed as the average number of transposon mutants/μg of gDNA. Values are averaged from triplicate independent determinations ± SD. “n.d.” indicates a transformation efficiency below the detection limit of the assay.