TABLE 1.
Transformation frequency and fold increase of Parvimonas micra isolatesa
| Parvimonas micra isolate | ATCC 33270 gDNA (rifR) | Transformation frequency (rifR/total CFU) (×10−4)b | Fold increase (over background) |
|---|---|---|---|
| A28 | + | 1.73 ± 0.34* | 752 |
| − | 0.0023 ± 0.0005 | ||
| A42 | + | 0.33 ± 0.12* | 236 |
| − | 0.0014 ± 0.0002 | ||
| A11 | + | 0.26 ± 0.06* | 263 |
| − | 0.00099 ± 0.00014 | ||
| ATCC 33270 | + | 0.075 ± 0.0029* | 36 |
| − | 0.0021 ± 0.0022 | ||
| A3 | + | 0.013 ± 0.00013* | 8 |
| − | 0.0016 ± 0.00043 | ||
| A1 | + | 0.0040 ± 0.0012n.s. | 1 |
| − | 0.0035 ± 0.0014 |
a
Transformation frequency is expressed as the number of rifampin-resistant bacteria/total CFU. +, indicates rifR and −, indicates WT gDNA. Values are averaged from triplicate determinations ± SD. Statistical analysis was performed using a two-tailed Student’s t test.
b
“*” indicates significance (P < 0.05) relative to WT gDNA control. “n.s.” indicates no significance (P > 0.60).