FIG 2.
Kinetic metabolic model reproduces independent experimental observations. (a) Electron fluxes from the oxidation to the reduction of the methyl-group in methanol. Values in parentheses show reduction potentials (V); arrow widths indicate electron fluxes relative to the flux of the reduction of methyl-coenzyme M to methane (i.e., 3.6 × 10−18 mol · s−1). (b) Gibbs free energy (ΔG) is unevenly distributed among enzyme reactions. (c) 81% of metabolites have concentrations greater than the respective Michaelis constants (Km). Solid line shows the 1:1 ratio; shaded area covers up to 10-fold deviations from the 1:1 ratio. See the Fig. 1 legend for definitions of abbreviations.