FIG 2.

Potent bactericidal activity and low toxicity of gcIFN-20 in vitro and in vivo. (A and B) Time-kill curves of gcIFN-20 (4×MBC₉₀) against G⁻ and G⁺ bacteria, respectively. The six bacterial strains are K. pneumoniae (ATCC 13883), P. aeruginosa (ATCC 9027), E. coli (ATCC 25922), S. pneumoniae (ATCC 49619), S. agalactiae (ATCC 13813) and S. aureus (ATCC 25923). (C) Hemolytic activity of gcIFN-20 was measured with SRBC, and melittin was used as the control. (D and E) The cytotoxicity of gcIFN-20 toward RAW 264.7 cells and Vero cells were examined by MTT assay, respectively. All the experiments were performed in triplicate. Data were presented as means ± SD.