FIG 4.

Potent bactericidal activity of gcIFN-20 in vivo. (A) Survival rates in mice were examined by injection with E. coli PCN033 and gcIFN-20 (n = 10). (B) Bacterial loads in mouse blood (CFU/mL), brain (CFU/g), and spleen (CFU/g) (gcIFN-20 25 μg/mouse) at 12 h post bacterial injection (n = 6). (C) IVIS analysis of the antimicrobial activity of gcIFN-20 (25 μg/mouse) in vivo against E. coli RS218. Bacterial loads were displayed in the image with an overlay of bioluminescence. False color imaging for strong luminescence was in red and mild luminescence in blue. The total flux was quantified by the IVIS software (n = 5). Data were presented as means ± SD. In vivo data were statistically analyzed via unpaired nonparametric Mann-Whitney U-test. * indicates P ≤ 0.01.