FIG 6.

Anti-inflammatory activity of gcIFN-20 in LPS-induced RAW 264.7 cells and mice. The productions of TNF-α (A) and NO (B) were investigated in RAW 264.7 cells, respectively. Dexamethasone serves as the control. (C) The protective effect of gcIFN-20 on lung tissue in an endotoxemia mouse model (n = 6). (D) The total lung lesion scores were generated in a blinded manner by a certified pathologist according to a semiquantitative scoring method. (E and F) The suppression of the LPS-stimulated TNF-α and IL-6 release in endotoxemia mice by gcIFN-20, respectively. (G) Immunohistochemical analysis of NF-κB p65 in the lung slices of experimental mice. All the experiments were performed in triplicate. Data were presented as means ± SD.