TABLE 2.

Analysis of productivity and selectivity properties of GVPC manufactured without O₂ and autoclaved at 115°C, 15 min (conditions 3 versus conditions 1) using real samples from cooling towers^a

Type of sample	Phaseb	GVPC Condition 1	GVPC Condition 3
		Interfering microorganisms (CFU/plate)	Interfering microorganisms (CFU/plate)
Water sample n°1	Phase A	0	0
	Phase B	30.00 ± 5.65	15.00 ± 1.41
	Phase C	22.00 ± 2.82	4.00 ± 2.82
	Phase D	32.50 ± 6.36	7.00 ± 2.82
Water sample n°1 + Legionella inoculation	Phase A	1.50 ± 0.70	0
	Phase B	34.50 ± 20.50	5.00 ± 2.82
	Phase C	0	0
	Phase D	17.00 ± 5.65	1
Water sample n°2	Phase A	0	0
	Phase B	5.50 ± 0.70	0
	Phase C	5.00	0
	Phase D	6.50 ± 3.53	0
Water sample n°2 + Legionella inoculation	Phase A	0	0
	Phase B	10.50 ± 0.70	0
	Phase C	7.50 ± 2.12	0
	Phase D	9.00 ± 1.41	0

^aColony count (CFU/plate) of interfering microorganisms.

^bPhase A, direct plating; phase B, plating of concentrated water from filtration; phase C, heat treatment (50°C, 30 min); phase D, acid treatment (HCl-ClK buffer, pH 2.2. for 5 min). The colony reading was performed after 5 incubation days at 37°C.