TABLE 3.

Analysis of productivity properties of GVPC manufactured without O2 and autoclaved at 115°C, 15 min (conditions 3 vs conditions 1) using real samples from cooling towers^a

Type of sample	Phaseb	CFU/L Legionella spp.
		GVPC Condition 1	GVPC Condition 3
Water sample n°1	Phase A	0	0
	Phase B	100	50
	Phase C	0	0
	Phase D	50	0
Water sample n°1 + Legionella inoculation	Phase A	0	0
	Phase B	100	50
	Phase C	0	0
	Phase D	0	0
Water sample n°2	Phase A	0	0
	Phase B	0	50
	Phase C	0	0
	Phase D	0	0
Water sample n°2 + Legionella inoculation	Phase A	0	50
	Phase B	200	300
	Phase C	50	0
	Phase D	50	0

^aLegionella colony count (CFU/L) of water samples from cooling waters.

^bPhase A, direct plating; Phase B, plating of concentrated water from filtration; Phase C, heat treatment (50°C, 30 min); Phase D, acid treatment (HCl-ClK buffer, pH 2.2. for 5 min). The colony reading was performed after 5 incubation days at 37°C.