FIG 1.

AmiL as a novel QS regulatory sRNA was identified by RNA-Seq. (A) The QS activation model, and growth curves of PAO1 and its lasI or rhlI gene-deficient strain (△lasI, △rhlI). (B) RNA-Seq study of PAO1 which was under QS activation (PAO1, t = 2 h, 4 h, 6 h) or QS inhibition (△lasI, △rhlI, t = 6 h) condition. (C) Volcano plot analysis of RNA-Seq results, the groups that PAO1 2 h versus 4 h and PAO1 6 h versus △lasI 6 h or △rhlI 6 h were showed with over 6,000 variables; AmiL, the black dot. (D) Genetic organization of the amiEBCRS operon, and transcription termination sequences are shown as a stem-loop symbol (T1, T2, T3).