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. 2022 Mar 9;10(2):e02211-21. doi: 10.1128/spectrum.02211-21

FIG 2.

FIG 2

The transcription of AmiL was probably inhibited by RhlR. (A, B) AmiL expression levels in QS activation and inhibition conditions were detected by qRT-PCR. The activation model of QS (t = 2 h, 4 h, 6 h) was used to analyze the expression of AmiL, lasI, and rhlI (A). At 6 h of growth, the expression levels of AmiL, lasI, rhlI in PAO1, and lasI or rhlI gene-deficient strain (△lasI, △rhlI) were shown, respectively (B). (C) After treatments with 3-oxo-C12-HSL or C4-HSL with concentrations ranging from 5 to 40 μM for 6 h, AmiL expression levels in PAO1 were measured by qRT-PCR, compared with the vehicle DMSO (0 μM). (D) Diagram of RhlR-binding motif in the operon of amiL, the putative target gene. (E) PAO1 carrying the pROp200 empty vector (EV), pROp200-lasR (LasR+), or pROp200-rhlR (RhlR+) was cultured in LB for 6 h, and AmiL expression was measured by qRT-PCR. Data are shown as mean ± SEM of at least three independent experiments. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, non-significant.