FIG 4.

AmiL positively regulated swarming motility, but had no impact on swimming. The strains, PAO1 carrying the pROp200 empty vector (EV) or pROp200-amiL (AmiL⁺), and amiL-deleted PAO1 carrying pROp200 (△AmiL) were used. (A) 5-μL cultures of the indicated strains were spotted onto the swarming medium and incubated for 16 h; swarming motility assays were performed. (B) 5-μL cultures of the indicated strains were spotted onto the swimming medium and incubated for 16 h; swimming motility assays were adopted. Data are shown as mean ± SEM of at least three independent experiments. **, P < 0.01; ***, P < 0.001; ns, non-significant.