FIG 7.

Schematic representation of a novel QS-sRNA regulatory network. The sRNA AmiL, negatively regulated by QS (probably RhlR), involves in regulating various QS-mediated virulence in PAO1, including pyocyanin, elastase, rhamnolipid, biofilm, swarming motility, hemolysin, and mammalian cytotoxicity. In addition to directly targeting PhzC, AmiL decreases lasI expression in the early growth of PAO1, but predominantly increased rhlI expression and C4-HSL production in the middle and late stages. Thus, this study clarified a novel signaling cascade of las/rhl (RhlR)-AmiL-PhzC/las/rhl in P. aeruginosa PAO1.