FIG 4.

Deletion of dhsphK1 impacts the lipid composition of P. gingivalis. (A) Total lipid extracts were analyzed for the relative levels of a serine-glycine lipid (lipid 1256) and various sphingolipid classes in the wild type (WT), the ΔdhsphK1 mutant (1348), and the complemented strain (Comp [pTCOW-1348]). The data represent the average values from three biological repeats. Number of repeats (n), 3. Error bars shown in the graph were calculated using the following formula: SE = SD/√n, where SE is the standard error of the sample, SD is the standard deviation of the sample, and n is the number of samples. (B and C) Donut plots for untargeted lipidomics for the wild type (W83) and the ΔdhsphK1 mutant showing (B) relative amounts of overall lipid classes and (C) relative amounts of overall lipid subclasses.