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. 2022 Apr 11;10(2):e01354-21. doi: 10.1128/spectrum.01354-21

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Copyright © 2022 Malhotra et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 6 — EMSAs of MtrA and PrrA binding in the presence of phosphorylation through cognate sensor kinase and PknK. (A and C) Analysis of the binding affinity of various amounts of RRs MtrA and PrrA with oriC DNA and prrA promoter region, respectively. (B and D) Analysis of the binding affinity of MtrA and PrrA RRs for their respective target DNAs in the presence of cognate SK, MtrB or PrrB, alone and in combination with STPK PknK. The SK or PknK wherever indicated is used in a phosphorylated state.