TABLE 3.
Primers used in this study
| Primer | Sequence (5′ to 3′) | Application | Reference |
|---|---|---|---|
| yiaDqPCR-F | TGGCTGAAGATAACAAGAGCGC | RT-qPCR for yiaD | This study |
| yiaDqPCR-R | CGATACGGCTAGATGGAACACC | ||
| 16S rRNA-F | CAGCTCGTGTCGTGAGATGT | RT-qPCR for 16S rRNA | This study |
| 16S rRNA-R | CGTAAGGGCCATGATGACTT | ||
| yiaDup-F | GTGATGAGTGCACACCTTAAAGTG | Construction of ΔyiaD::kan | This study |
| yiaDup-R | TCCAGCCTACACAATCATTGCATGCCTCCT | ||
| yiaDdw-F | GGAGGATATTCATATGGACCTAAGATCTAGTT | Construction of ΔyiaD::kan | This study |
| yiaDdw-R | GGCAGATGACATCGTGTATTACC | ||
| kanFRT-F | ATTGTGTAGGCTGGAGCTGCTTC | Construction of ΔyiaD::kan | This study |
| kanFRT-R | GGTCCATATGAATATCCTCCTTAGTTCC | ||
| yiaDscreen-F | GGCCCCGAATATCCAAAATCC | Confirmation of ΔyiaD::kan | This study |
| yiaDscreen-R | CCAGCAAGCACCAATAAAGGG | ||
| yiaDcomp-F | GGTACCGTTGACATATAAATGGCCCC | Complementation of yiaD | This study |
| yiaDcomp-R | GTCGACAAAACCACCCGAAGGT | ||
| blaKPC-F | TGTCACTGTATCGCCGTC | Detection of blaKPC | (59) |
| blaKPC-R | CTCAGTGCTCTACAGAAAACC | ||
| blaGES-F | GTTTTTGCAATGTGCTCAACG | Detection of blaGES | (60) |
| blaGES-R | TGCCATAGCAATAGGCGTAG | ||
| blaTEM-F | ATAAAATTCTTGAAGACGAAA | Detection of blaTEM | (60) |
| blaTEM-R | GACAGTTAGCAATGCTTAATCA | ||
| blaSHV-F | GCCTTTATCGGCCCTCACTCAAG | Detection of blaSHV | This study |
| blaSHV-R | TTAGCGTTGCCAGTGCTCGATCA | ||
| blaCTX-F | CGTCACGCTGTTGTTAGGAA | Detection of blaCTX | (60) |
| blaCTX-R | ACCGTCGGTGACGATTTTAG | ||
| blaIMP-F | GAAGGCGTTTATGTTCATAC | Detection of blaIMP | (61) |
| blaIMP-R | GTACGTTTCAAGAGTGATGC | ||
| blaVIM-F | GTTTGGTCGCATATCGCAAC | Detection of blaVIM | (61) |
| blaVIM-R | AATGCGCAGCACCAGGATAG | ||
| blaSIM-1-F | TACAAGGGATTCGGCATCG | Detection of blaSIM-1 | (60) |
| blaSIM-1-R | TAATGGCCTGTTCCCATGTG | ||
| blaNDM-F | GCAGCTTGTCGGCCATGCGGGC | Detection of blaNDM | (61) |
| blaNDM-R | GGTCGCGAAGCTGAGCACCGCAT | ||
| blaOXA-23-like-F | GATGTGTCATAGTATTCGTCG | Detection of blaOXA-23-like | (60) |
| blaOXA-23-like-R | TCACAACAACTAAAAGCACTG | ||
| blaOXA-24-like-F | GGTTAGTTGGCCCCCTTAAA | Detection of blaOXA-24-like | (60) |
| blaOXA-24-like-R | AGTTGAGCGAAAGGGGATT | ||
| blaOXA-48-like-F | GCGTGGTTAAGGATGAACAC | Detection of blaOXA-48-like | (61) |
| blaOXA-48-like-R | CATCAAGTTCAACCCAACCG | ||
| blaOXA-51-like-F | ATGAACATTAAAGCACTC | Detection of blaOXA-51-like | (60) |
| blaOXA-51-like-R | CTATAAAATACCTAATTGTTC | ||
| blaOXA-58-like-F | AAGTATTGGGGCTTGTGCTG | Detection of blaOXA-58-like | (60) |
| blaOXA-58-like-R | CCCCTCTGCGCTCTACATAC | ||
| TEMqPCR-F | TTCCGGCTGGCTGGTTTATT | RT- qPCR for TEM | This study |
| TEMqPCR-R | TGACTCCCCGTCGTGTAGAT | ||
| OXA-23-likeqPCR-F | TAATGCTCTAAGCCGCGCAA | RT-qPCR for OXA-23-like | This study |
| OXA-23-likeqPCR-R | TTCTCCAATCCGATCAGGGC | ||
| OXA-51-likeqPCR-F | TCGGCCTTGAGCACCATAAG | RT-qPCR for OXA-51-like | This study |
| OXA-51-likeqPCR-R | GCCATAACCAACACGCTTCA |