FIG 4.

Native MS investigation of the dimerization of PA2880. (a) The LILBID spectra of the wild-type (wt) PA2880 with/without fusion of eGFP. All measurements were performed at pH 8. (b) The pH dependence of the dimerization of all aforementioned PA2880 variants. No strong effects of pH on protein dimerization were detected. The dimer ratio was calculated from deconvoluted zero-charge spectra, taking the overlap of different charge states of monomer and dimer into account. (c) The spectra of the PA2880 wild type and the E38Q variant. All measurements were performed at pH 8. (d) PA2880 dimer population in the presence of chlorhexidine, indicating that chlorhexidine strengthens the dimer formation.