Table 1. Primers used for detection of blaKPC and blaNDM carbapenemase genes in K. pneumoniae isolates.
Gene | Primer | Nucleotide sequence | Annealing Temp °C | Amplicon size (bp) | Reference | |
---|---|---|---|---|---|---|
5`————————-3` | ||||||
bla KPC | KPC | F | CGTCTAGTTCTGCTGTCTTG | 57.8 | 798 | [15] |
R | CTTGTCATCCTTGTTAGGCG | 62.2 | ||||
bla NDM | NDM | F | GGTTTGGCGATCTGGTTTTC | 65.5 | 621 | |
R | CGGAATGGCTCATCACGATC | 67.8 |