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. 2022 Apr 27;17(4):e0267657. doi: 10.1371/journal.pone.0267657

Table 1. Primers used for detection of blaKPC and blaNDM carbapenemase genes in K. pneumoniae isolates.

Gene Primer Nucleotide sequence Annealing Temp °C Amplicon size (bp) Reference
5`————————-3`
bla KPC KPC F CGTCTAGTTCTGCTGTCTTG 57.8 798 [15]
R CTTGTCATCCTTGTTAGGCG 62.2
bla NDM NDM F GGTTTGGCGATCTGGTTTTC 65.5 621
R CGGAATGGCTCATCACGATC 67.8